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38

Films of enzymes

(Alkaline phosphatase; LS technique of deposition;
Spectrophotometric method of activity measurement; [10])

Enzymatic activity was determined by the hydrolysis of p-nitrophenilphosphate. Production of p-nitrophenol was monitored at a wavelength of 405 nm. Specific activity was evaluated to be of about 1% of solution activity (10 μmol min-1 mg-1 at 25C).

 

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Surface pressure                   Enzymatic activity (μmol min-1cm-2)

     (mN m-1)                          25C                             80C

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         35                                  0.00197                         0.00131

         45                                  0.00395                         0.00197

         50                                  0.0054                           0.0006

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