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                    Activity of enzymes:

Film on the basis of GST

Assay conditions: 0.1 M phosphate buffer, pH 6.5, 25C; 0,001 M EDTA; 1.0 mM 1-Chloro-2,4-dinitrobenzene; 2.5 mM GSH

1 - solution (slope 106 10-5 1/s), 2 - proposed method (the best, slope 90 10-5 1/s), 3 - proposed method (average, slope 53 10-5 1/s), 4 - Langmuir-Schaefer method (the best, slope 6 10-5 1/s), 5 - spontaneneous reaction (average, slope 16.4 10-5 1/s). Curves 1-4 show the difference of absorbances for catalized and spontaneous reactions.

Optical microscopy and interference studies:

"Superlattice" based on Cyt P450 (10 periods)

a-morphology,b-interference pattern (thickness of C. P450 layer is calculated to be 5 nm)

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